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Techniques

Light microscopy at CIUS (German)

Microscopy

Microscopy is our main tool to investigate cells and their contents. Routinely, we use all contrast methods in light microscopy like differential interference contrast, phase contrast, dark field and polarisation. Submicroscopical structures are visualised in fluorescence and confocal laser scan microscopy. Due to our focus on dynamic processes in living cells, we are also well equipped with video and film techniques for documentation and analysis through the microscope.

> Light microscopy
> Fluorescence microscopy
> Video and UV microscopy
> Confocal laser scanning microscopy
> Micromanipulation and microinjection

If you intend to use the instruments in our lab, please contact Dr. Ingeborg Lang for further information and registration. If you are already a registered user, you can book the instruments online via Webcoach.

> Microscopy equipment

  

Preparation at CIUS (German)

Preparation

Observation in the LM often requires sectioning of the sample with a microtome. Therefore, fixation and embedding may be needed. In addition, staining with organelle-specific fluorescent dyes and with antibodies as well as FISH techniques need fixation of the cytoplasm which is either possible by chemical substances or by freezing. Fixed cells can be further processed for observation in the electron microscope.

> Preparation pathways

> Preparation techniques

If you intend to use the preparation instruments in our lab, please contact Dr. Ingeborg Lang for further information and registration. If you are already a registered user, you can book the instruments online via Webcoach.

> Preparation equipment

  

Preparation pathways at CIUS
Scientific documentation at CIUS (German)

Scientific documentation and visualization - photography, video, media design

With our scientific documentation and visualization tools we do macro photography and video, HDR photography, time lapse photography, panorama and object movie photography, animations and media design.

If you intend to use our scientific documentation and visualization equipment, please contact Gregor Eder for further information and registration.

> Scientific documentation and visualization techniques

> Scientific documentation and visualization equipment

  

Electron microscopy

In order to visualize submicroscopic structures, we provide scanning-(SEM) and transmission electron microscopes (TEM) as well as associated equipment for sample preparation. In addition, we are equipped for spectrometric techniques (EDX, EELS) to study the elemental composition of biological samples. We also adapt and refine cryo fixation and dehydration methods for various tissue types.

Electron microscopes surpass the resolution of visible light which is limited to about 300 nm (wavelength/2). Electrons can be excited to much higher energies, corresponding to shorter wave lengths. This enables spatial resolutions of about one nanometer. The electrons are emitted by a cathode with a high voltage applied (1-300 kV), deflected by electromagnetic lenses (coils) and limited by apertures to create a controllable beam.

This advantage of SEM and TEM (high resolution, possibility for element analyses) has to be paid with higher effort (need of dry samples, high vacuum, and extensive sample preparation).

Scanning electron microscopy (SEM)

Transmission electron microscopy (TEM)

Spectroscopic methods (EDX, EELS)

Cryo preparation

 

 

 

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Lastupdate: 09.04.2018 - 09:24