Electron Microscopy

In order to visualize submicroscopic structures, we provide scanning-(SEM) and transmission electron microscopes (TEM) as well as associated equipment for sample preparation. In addition, we are equipped for spectrometric techniques (EDX, EELS) to study the elemental composition of biological samples. We also adapt and refine cryo fixation and dehydration methods for various tissue types.

Electron microscopes surpass the resolution of visible light which is limited to about 300 nm (wavelength/2). Electrons can be excited to much higher energies, corresponding to shorter wave lengths. This enables spatial resolutions of about one nanometer. The electrons are emitted by a cathode, accelerated by high voltage (1-300 kV), deflected by electromagnetic lenses (coils) and limited by apertures to create a controllable beam.

This advantage of SEM and TEM (high resolution, possibility for element analyses) has to be paid with higher technical effort (need of dry samples, high vacuum, and extensive sample preparation).

> Scanning electron microscopy (SEM)

> Transmission electron microscopy (TEM)

> Spectroscopic methods (EDX, EELS)

> Cryo preparation

> Immuno labeling

If you intend to use the preparation instruments in our lab, please contact Mag. Daniela Gruber or Dr. Norbert Cyran for further information and registration. If you are already a registered user, you can book the instruments online via Webcoach.

> EM Preparation equipment

Electron microscopes