Cutting and Slicing

Leica UC7 - Ultramicrotome (2x)

  • High-quality ultrathin sectioning
  • Thickness feed range from 30 nm to 5 µm
  • Cutting speed 0.05 - 100 mm/s
  • Fully motorized knife stage
  • Improved illumination
  • Auto-trim function
  • Ergonomic touch screen
  • Can be used with the cryochamber Leica FC7 for cryosectioning

Leica Cryochamber FC7 - for cryosectioning

  • For cryosectioning of vitrified samples (Tokuyasu method for immunogold labeling)
  • Full control integration with the EM UC7 control panel
  • Highly stable temperature regulation by LN2
  • Electrostatic charge and discharge functions for section adherence (EM CRION ionizer)

Leica UC6 - Ultramicrotome

  • High-quality ultrathin sectioning
  • Thickness feed range from 30 nm to 5 µm
  • Cutting speed 0.05 - 100 mm/s
  • Fully motorized knife stage
  • Improved illumination

Leica UCS - Ultramicrotome

  • Sectioning thin and ultra-thin sections for light- and electron microscopy
  • Thickness feed range from 30 nm to 5 µm
  • Cutting speed 0.05 - 100 mm/s
  • Fully motorized knife stage

Leica VT1200 - Vibratome

  • Cutting frequency 85 Hz (± 10 %)        
  • Amplitude 0 - 3 mm in 0,05 mm-steps     
  • Cutting speed 0.01 - 1.5 mm/s (± 10 %)                    
  • Maximum vertical sample lift 20 mm (motorised)
  • Cutting range 45 mm (motorised)           

Leica KMR3 - Knifemaker

  • Manufacturing of glass knives for ultramicrotomy  
  • For glass stripes with 6.4 mm, 8 mm or 10 mm thickness

Sample preparation

Leica CPD 300 - Critical point dryer

  • Drying samples for SEM examination by replacement of organic solvent with liquid CO2 and subsequent heating until CO2 is sublimated at the critical point       
  • Excellent structural preservation of samples

JEOL JFC-2300HR - Sputter coater

  • Coating SEM samples with a conductive metal layer (Au, Pt, Cr, ...)
  • Sample plate for up to 24 samples, rotatable by a planetary gear for a convenient charging
  • Sputtering by time in sec or by coating thickness measurement

Quorum GloQube - Glow discharge

  • Hydrophilisation of surfaces (glass slides, TEM grids with or without carbon support films, ...) for better sample spreading --> Negative and positive discharge modes!
  • Improved adhesion and orientation of proteins, nucleic acids, nanoparticles and antibodies to support films and substrates
  • Intuitive touch screen control
  • Fully automatic procedure

Pelco BioWave Pro - Microwave oven

  • Microwave Power: 100 - 750 watts
  • Microwave Frequency: 2.45 Ghz
  • Temperature Probe: ±1°C
  • Extern steady temperature unit
  • Cold spot tray
  • Integrated magnetic stirrer, 0 - 3000rpm speed
  • Vacuum System: 20" Hg
  • User Interface: 7" Touchscreen

Memmert UF55 plus - Polimerisation oven (2x)

  • Adjustable from 20 - 300°C
  • Modes:
    • Permanent
    • Backward timer
    • Creating user defined time programs on your own computer - EXAMPLE
  • Air circulation, adjustable in 10 steps
  • Vent connection leading in the house extraction system
  • TFT-color display
  • USB port for data transfer

Cryoinstruments

Leica HPM 100 - High pressure freezer

  • Vitrification of hydrated samples within milliseconds at 2100 bar
  • Sample carriers: 3 or 6 mm in diameter, up to 300 µm in depth
  • In combination with a long-distance stereomicroscope for sample dissection and mounting
  • Automated sample transfer into LN2

Leica EM-CPC - Plunge freezer

  • Immersion cryofixation in liquid propane (or ethane on request)
  • Mobile working platform for on-site use

Leica AFS 2 - Freeze substitution

  • Automated freeze substitution of frozen-hydrated samples resulting in low-temperature dehydration and fixation
  • Temperature / time schedules programmable
  • Equipped with an agitation module for accelerated processing
  • LED lamp for UV-polymerisation of low-temperature resins (Lowicryls)

Leica EM MED 020 - Freeze dryer and Carbon coater

  • Carbon coating by carbon thread evaporation
    • EDX analysis on the SEM
    • Carbon reinforcement film on formvar coated TEM grids
  • Freeze drying under high vacuum conditions (sublimation)
    • External LN2 pumping device
    • Temperature / time schedules programmable